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Barbara Diaz-Rohrer on Muck Rack

Barbara Diaz-Rohrer

As seen in: Wiley Online Library, PLOS Biology, BioRxiv, eLife
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Articles

Live Cell Painting: image-based profiling in live cells using Acridine Orange

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By Barbara Diaz-Rohrer, Fernanda Garcia-Fossa, Thais Moraes-Lacerda, Mariana Rodrigues-da-Silva
| BioRxiv
Abstract Image-based profiling has been used to analyze cell health, drug mechanism of action, CRISPR-edited cells, and overall cytotoxicity. Cell Painting is a broadly used image-based assay that uses morphological features to capture how cells respond to treatments. However, this method requires cell fixation for staining, which prevents examining live cells.
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Partitioning to ordered membrane domains regulates the kinetics of secretory traffic

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By Ivan Castello-Serrano, Frederick A. Heberle, Barbara Diaz-Rohrer, Rossana Ippolito
| eLife
eLife assessment In this important study, Castello-Serrano and colleagues describe, model and quantify the role of transmembrane domains in protein sorting in the secretory pathway, first at the ER and subsequently at the Golgi. Convincing data support the role of a cytoplasmic motif in ER exit, while further experiments are necessary to support a direct connection between the phase partitioning capability of the transmembrane regions and the sorting potential of domains at the Golgi/TGN.
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CellProfiler plugins - An easy image analysis platform integration for containers and Python tools

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By Erin Weisbart, Callum Tromans-Coia, Barbara Diaz-Rohrer, David Stirling
| Wiley Online Library Verified
CONFLICT OF INTEREST STATEMENT The authors declare that there are no competing interests associated with the manuscript. REFERENCES 1, , , , , , , & (2019). Scientific Community Image Forum: A discussion forum for scientific image software. PLoS Biology, 17, e3000340. 2, , , , , , , , , & (2022). A Hitchhiker's guide through the bio-image analysis software universe. Febs Letters, 596, 2472–2485. 3, , , , , , , , , , , & (2021).
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