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Metagenomic Detection of Divergent Insect- and Bat-Associated Viruses in Plasma from Two African Individuals Enrolled in Blood-Borne Surveillance
Abstract : Metagenomic next-generation sequencing (mNGS) has enabled the high-throughput multiplexed identification of sequences from microbes of potential medical relevance. This approach has become indispensable for viral pathogen discovery and broad-based surveillance of emerging or re-emerging pathogens. From 2015 to 2019, plasma was collected from 9586 individuals in Cameroon and the Democratic Republic of the Congo enrolled in a combined hepatitis virus and retrovirus surveillance program.
Hepatitis B genotypes and surface antigen mutants present in Pakistani blood donors
For this study, HBsAg reactive specimens, identified by routine blood-screening testing, were collected at the Armed Forces Institute of Transfusion (AFIT) and Hussaini Blood Bank (HBB) from 2009–2013. AFIT, a regional blood center located in northern Pakistan in the Punjab province, serves the armed forces hospitals in Rawalpindi and Islamabad, and civilian hospitals in the region [ 13 ].
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