Barbara M. Bakker

Abbreviations aX thermodynamic activity of chemical species X = γ X · X $$ {\gamma}_X\cdotp \left[X\right] $$ ATP adenosine 5’ triphosphate ADP adenosine 5’ diphosphate AMP adenosine 5’ monophosphate BDH constant related to the diameter of the central ion in the Debye-Hückel theory cj volumetric concentration of chemical species j °C degrees Celsius CO2 carbon dioxide D2O Deuterium oxide e 2.71828 (Euler's number, the base of the natural logarithms) EDTA ethylenediamine tetra-acetic acid FAIR...

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Abstract Fasting hypoglycemia is a severe and incompletely understood symptom of various inborn errors of metabolism (IEM). Precision-cut liver slices (PCLS) represent a promising model for studying glucose production ex vivo. This study quantified the net glucose production of human and murine PCLS in the presence of different gluconeogenic precursors. Dihydroxyacetone-supplemented slices from the fed mice yielded the highest rate, further stimulated by forskolin and dibutyryl-cAMP.