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Traces of Canine Inflammatory Bowel Disease Reflected by Intestinal Organoids
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Bevacizumab Efficiently Inhibits VEGF-Associated Cellular Processes in Equine Umbilical Vein Endothelial Cells: An In Vitro Characterization
2. Materials and Methods 2.1. Drug Preparation Bevacizumab 25 mg/mL (Avastin®) was obtained from Roche (Basel, Switzerland). Solutions of different concentrations (1, 2, 4, 6, and 8 mg/mL) of bevacizumab were aseptically prepared in compliance with good manufacturing practice with sterile 0.9% saline solution as a solvent. Cell culture medium was used as a control (Ctrl, 0 mg/mL bevacizumab).
The Intricacies of Inflammatory Bowel Disease: A Preliminary Study of Redox Biology in Intestinal Organoids
1. Introduction In vitro cultured organoids can model the physiological and morphological characteristics as well as cell signaling pathways within humans or animals. Gastrointestinal organoid cultures are most commonly derived from adult stem cells (ASC) that reside in the crypts of the gastrointestinal tract. The stem cells can be isolated from the tissue and retain their function and mutations from their residence site in the gut when maintained under defined cell culture conditions [1].
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