Barbara Rolfe

Malignant pleural mesothelioma (MPM) is a rare and aggressive thoracic cancer that derives from the mesothelial cells of the pleura and is causally associated with exposure to asbestos. Because of the poor specificity of the clinical symptoms, when it is diagnosed, malignant cells, which are extremely resistant to therapies, have already spread throughout the pleural layers, leading to a poor outcome.

Yunhao Bai1,2†, Bokai Zhu1,3†, Xavier Rovira-Clave1, Han Chen1, Maxim Markovic1, Chi Ngai Chan4, Tung-Hung Su1,5, David R. McIlwain1, Jacob D. Estes4, Leeat Keren1,6, Garry P.

Introduction High-dimensional tissue imaging approaches such as CODetection by indEXing (CODEX), Multiplexed Ion Beam Imaging (MIBI), Cyclic Immunofluorescence (CyCIF), and imaging mass cytometry have contributed to our understanding of tissue biology and microenvironmental changes in disease (1–7). These methods, which are based on either fluorescence or isotope detection, retain the tissue context of single cells while enabling deep phenotyping capabilities (8).