Barbara Schamberger

Abstract Little is known about the contribution of 3D surface geometry on the development of multi-layered tissues containing fibrous extracellular matrix components such as those found in bone. Here we elucidate the role of curvature in the formation of chiral, twisted plywood-like structures. Tissues consisting of murine pre-osteoblast cells (MC3T3-E1) were grown on 3D scaffolds with constant mean curvature and negative Gaussian curvature for up to 32 days.

1 Introduction 3D additive manufacturing strategies are among the most prominent technologies for microfabricating polymer-based scaffolds, with high accuracy and defined morphologies.[1]Among these strategies, 2-photon polymerization (2PP) utilizes femtosecond lasers to polymerize liquid polymers, enabling the creation of precise structures at micro- and even nano-scale.[2] As many cell types interact with three-dimensional environments, 2PP is a promising technique for creating complex 3D...