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Regulation of claudin/zonula occludens-1 complexes by hetero-claudin interactions
Animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee of Emory University. Adult male Sprague–Dawley rats were pair-fed ethanol (36% of total calories) or control isocaloric maltin-dextrin using the liquid Lieber DeCarli Diet (Research Diets, New Brunswick, NJ) ad libitum for 6–8 weeks4. Animal use was limited to their use as a source for primary cells and thus sample size and randomization are not relevant variables.
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