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Baronissi Salerno on Muck Rack

Baronissi Salerno

As seen in: Biomed Central (BMC)
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Articles

Healthy longevity-associated protein improves cardiac function in murine models of cardiomyopathy with preserved ejection fraction - Cardiovascular Diabetology

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By Khaled Kassem, Annibale Alessandro, Valeria Vincenza, Baronissi Salerno
| Biomed Central (BMC)
Hek-293 cells were transfected with the LAV-BPIFB4 vector cloned in fusion with His-Tag. The recombinant protein was purified using affinity Nuvia IMAC Resin (Bio-Rad). We used the eluate of cells transfected with the null vector as a control. [23] Two controlled, randomized studies followed the EU Directive 2010/63/EU and the principles stated in the Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal Resources, 1996).
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