Barry Lutz
Seattle
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Immunomagnetic Sample Preparation Targeting Host- and Viral-Derived Antigens for HIV-1 Isolation from Limited Plasma Volumes Click to copy article link Article link copied!
Introduction Click to copy section linkSection link copied! Approximately 39.9 million people worldwide were living with HIV (PLHIV) in 2023, with 14% unaware of their status. (1) Early diagnosis during acute infection (<3 months postinfection), when HIV antibodies are absent and only HIV RNA or p24 antigen is detectable, is critical because individuals in this stage account for 10–50% of new transmissions due to their undiagnosed status and high viral loads (VLs).
Immunomagnetic Sample Preparation Targeting Host- and Viral-Derived Antigens for HIV-1 Isolation from Limited Plasma Volumes Click to copy article link Article link copied!
Introduction Click to copy section linkSection link copied! Approximately 39.9 million people worldwide were living with HIV (PLHIV) in 2023, with 14% unaware of their status. (1) Early diagnosis during acute infection (<3 months postinfection), when HIV antibodies are absent and only HIV RNA or p24 antigen is detectable, is critical because individuals in this stage account for 10–50% of new transmissions due to their undiagnosed status and high viral loads (VLs).
Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples
Loading metrics Open Access Peer-reviewed Research Article Amy Oreskovic, Barry R. Lutz Amy Oreskovic, Barry R. Lutz Published: February 26, 2021 https://doi.org/10.1371/journal.pone.0247851 Figures Abstract Urine cell-free DNA (cfDNA) is a valuable non-invasive biomarker with broad potential clinical applications, but there is no consensus on its optimal pre-analytical methodology, including the DNA extraction step.
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