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Bart W. Bardoel on Muck Rack

Bart W. Bardoel

As seen in: Nature, PLOS
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Articles

Agnostic B cell selection approach identifies antibodies against K. pneumoniae that synergistically drive complement activation - Nature Communications

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By Bart W. Bardoel, Renoud J. Marijnissen, Janine Schuurman, Priscilla F. Kerkman, Stan Baijens, Remy M. Muts, Johannes Preiner
| Nature Verified
Abstract Antibody-dependent complement activation plays a key role in the natural human immune response to infections. Currently, the understanding of which antibody-antigen combinations drive a potent complement response on bacteria is limited. Here, we develop an antigen-agnostic approach to stain and single-cell sort human IgG memory B cells recognizing intact bacterial cells, keeping surface antigens in their natural context. With this method we successfully identified 29 antibodies against K.
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Correction: Polymerization of C9 enhances bacterial cell envelope damage and killing by membrane attack complex pores

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By Dennis J. Doorduijn, Maartje Ruyken, Piet Aerts, Bart W. Bardoel, PLOS Pathogens
| PLOS
Fig 2 is incorrect. The x-axis labels for C8 + C9TMH-1 lock and C8 + C9wt + DTT are incorrectly switched in panel B. The authors have provided a corrected version of Fig 2 here. Fig 2. Polymerization of C9 enhances bacterial killing by MAC pores. E. coli MG1655 was labelled with C5b-7 by incubating in 10% C8-depleted serum for 30 minutes. Bacteria were washed and next incubated with 10 nM C8 for 15 minutes.
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