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Abstract Retinoblastoma (RB) is the most common intraocular malignancy in children due to inactivation of the RB1 gene, and delayed diagnosis often results in poor outcomes due to its aggressive metastatic potential. Membrane potential (MP) has been implicated in tumor invasion, yet the upstream signaling pathways shaping RB bioelectricity remain unknown.
2.2 Oxidative stress is increased in rd10 retinas To confirm that oxidative stress is an important pathological mechanism in the animal model used in this study, we have analyzed various markers involved in oxidative stress, including the expression of hydroxynonenal (HNE), reduced and oxidized glutathione (GSH-GSSG), and the catalytic subunit of Glutamate cysteine ligase catalytic (GCLC) enzyme in retinal sections from rd10 animals at P18.
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