Is this you? As a journalist, you can create a free Muck Rack account to customize your profile, list your contact preferences, and upload a portfolio of your best work.
Claim your profile
Articles by Benjamin Demaree
Microbiome Single Cell Atlases Generated with a Commercial Instrument
1 Introduction Microbiomes, composed of diverse microorganisms, such as bacteria, archaea, fungi, and virus, are highly complex, often containing thousands of species and billions of cells even in small samples.[1] Beyond species diversity, they exhibit significant genetic heterogeneity, driven by differences in core genomes and mobile genetic elements like plasmids and transposons.[2-4] These mobile elements influence microbial properties and ecosystem dynamics by spreading traits such as...
Microbiome single cell atlases generated with a commercial instrument
Abstract Single cell sequencing is useful for resolving complex systems into their composite cell types and computationally mining them for unique features that are masked in pooled sequencing. However, while commercial instruments have made single cell analysis widespread for mammalian cells, analogous tools for microbes are limited. Here, we present EASi-seq (Easily Accessible Single microbe sequencing).
Joint profiling of DNA and proteins in single cells to dissect genotype-phenotype associations in leukemia
Abstract Studies of acute myeloid leukemia rely on DNA sequencing and immunophenotyping by flow cytometry as primary tools for disease characterization. However, leukemia tumor heterogeneity complicates integration of DNA variants and immunophenotypes from separate measurements.
Single-cell mutation analysis of clonal evolution in myeloid malignancies
Abstract Myeloid malignancies, including acute myeloid leukaemia (AML), arise from the expansion of haematopoietic stem and progenitor cells that acquire somatic mutations. Bulk molecular profiling has suggested that mutations are acquired in a stepwise fashion: mutant genes with high variant allele frequencies appear early in leukaemogenesis, and mutations with lower variant allele frequencies are thought to be acquired later1,2,3.
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
The SiC-seq experimental workflow contains 3 PDMS microfluidic devices fabricated using a soft lithography procedure (Figure 1). A co-flow dropmaker (Figure 3A) generates 25 µm of digital barcode droplets for labeling genomic DNA with a unique single-cell identifier. The barcode oligonucleotides consist of a 15 bp degenerate sequence flanked by PCR handles for amplification (Table 2, BAR primer).
Show More
loading
Actions
Is this you?
As a journalist, you can create a free Muck Rack account to customize your profile, list your contact preferences, and upload a portfolio of your best work.Get in touch with Benjamin
Contact Benjamin, search articles and posts on X, monitor coverage, and track replies from one place.
Learn more about Muck Rack