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Abstract Evolutionary relationships among homologous enzymes can be leveraged to introduce large numbers of amino acid substitutions for improved functional properties. Here, we combine evolution-informed design (EVdesign) with rational design to engineer improved poly(ethylene terephthalate) (PET) hydrolases (PETases).
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There are many billions of chronic viral infections in humans, as each of the ~7.4 billion individuals alive today each host ~10 chronic infections. The clinical significance of these infections vary widely, yet all individuals with chronic viral infections rely on persistent antiviral immunity to keep these infections in check.
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