Bernhard Voelkl

Loading metrics Open Access Peer-reviewed Meta-Research Article ? This is an uncorrected proof. Abstract The influence of protocol standardization between laboratories on their replicability of preclinical results has not been addressed in a systematic way.

Nuclei isolation and fluorescence-activated nuclei sorting (FANS). The ATAC-seq analysis was performed on ventral hippocampi isolated from male mice at TP1 (PND 56) and TP2 (PND 102). For this, test-naïve male mice were used to avoid effects of testing on the chromatin profile. Five cages per RF were selected randomly from all cages containing 3 male littermates after weaning.

“We demand rigidly defined areas of doubt and uncertainty!” —D. Adams Fig. 1: A confounding factor can be ignored, standardized or heterogenized. Fig. 2: Standardization increases power but leads to higher variation between labs if an interaction between treatment and confounder is present. Fig. 3: Confounder interaction can increase power to detect a treatment effect but reduce reproducibility. References 1. Würbel, H. Nat. Genet. 26, 263 (2000). Article Google Scholar 2. Krzywinski, M. & Altman, N. Nat.