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Biswarathan Ramani

As seen in: Nature, BioRxiv
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Articles

CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes - Nature Neuroscience

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By Biswarathan Ramani, Spencer Danner-Bocks, Ruilin Tian, Jorge J. Palop
| Nature Verified
Abstract There is a substantial need for scalable CRISPR-based genetic screening methods that can be applied in mammalian tissues in vivo while enabling cell-type-specific analysis. Here we developed an adeno-associated virus (AAV)-based CRISPR screening platform, CrAAVe-seq, that incorporates a Cre-sensitive sgRNA construct for pooled screening within targeted cell populations in mouse tissues.
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CRISPR screening by AAV episome-sequencing (CrAAVe-seq) is a highly scalable cell type-specific in vivo screening platform

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By Biswarathan Ramani, Noam Teyssier, Andrew J Pan, Spencer Danner-Bocks
| BioRxiv
Abstract There is a significant need for scalable CRISPR-based genetic screening methods that can be applied directly in mammalian tissues in vivo while enabling cell type-specific analysis. To address this, we developed an adeno-associated virus (AAV)-based CRISPR screening platform, CrAAVe-seq, that incorporates a Cre-sensitive sgRNA construct for pooled screening within targeted cell populations in the mouse tissues.
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Scalable, cell type-selective in vivo AAV-based CRISPR screens in mice

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By Biswarathan Ramani, Andrew J Pan, Ruilin Tian, Keran Ma
| BioRxiv
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