Bong Hyun Sung

To develop a screening system for MDH mutants with increased formaldehyde reduction activity, we exploited the cytotoxicity differences between formaldehyde and methanol in Escherichia coli. The minimal inhibitory concentration (MIC) of formaldehyde in E. coli is approximately 5 mM21, while that of methanol is >3 M22. When we treated E. coli BL21 (DE3) cells expressing wild type MDH with various concentration of formaldehyde, cell growth was arrested at concentrations >2 mM (data not shown).

S. Kim, B. H. Sung, S. C. Kim and H. S. Lee, Chem. Commun., 2018, Accepted Manuscript , DOI: 10.1039/C8CC00281A If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using RightsLink. Go to our Instructions for using RightsLink page for details.