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Results To select for potentiated Hsp104 variants, we co-expressed a library of randomized MD variants in yeast with TDP-43 (Figures 1A and 1B). Here, galactose induces expression of both TDP-43 and Hsp104, and yeast harboring suppressors of TDP-43 toxicity display a growth advantage.26,28,29 We harvested DNA from yeast populations before and after selection and sequenced the MD regions using the Illumina MiSeq platform.
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