Bryan Conway

Abstract MicroRNAs may act as diagnostic and prognostic biomarkers of chronic kidney disease and are functionally important in disease pathogenesis. To identify novel microRNA biomarkers, we performed small RNA-sequencing on plasma from individuals with type 2 diabetes, with and without chronic kidney disease. MiR-190a-5p abundance was significantly lower in the circulation of type 2 diabetic patients with reduced function compared to those with normal kidney function.

Significance Statement The innate immune system is central to injury and repair in the kidney, but the heterogeneity of myeloid cell subsets behind these processes is unknown. Complementary technologies—including bulk tissue transcriptomics, integrated droplet– and plate-based single-cell RNA sequencing, and paired blood exchange—resolved myeloid cell heterogeneity in a murine model of reversible unilateral ureteric obstruction, creating a single-cell atlas.

f11photo/Shutterstock This article was originally written in 1938 as told by Bryan Conway, No. 293 to T.H. Alexander and appeared in the April 1938 issue of Reader’s Digest.* ONE WHO has just finished, as I have, a 12-year stretch for murder generally tries to soften the facts in his record. Personally, I have no alibi to advance. I killed an Army sergeant to protect my own life. I served ten years in Atlanta Federal Penitentiary, which was bad enough, and 2.0 months in Alcatraz, which was worse.