Bumpei Samata

During early development of mouse brain, LMX1A is expressed in midbrain14, whereas CORIN is expressed in FP9. Based on these findings, we hypothesized that VM cells including mDA progenitors can be purified by a combination of these two markers (Fig. 1a–c). To visualize midbrain cells, we produced an LMX1A::GFP KI mESC line. The cells were induced to differentiate into neuronal lineage by the serum-free floating culture of embryoid body-like aggregates with the quick reaggregation method (Fig. 1d)15.