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Finnish-specific AKT2 gene variant leads to impaired insulin signalling in myotubes

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By Byrne CD, Moore CE, Cagampang FR, Stead RL
| bioscientifica.com
Figure 8 MNK2 and FAK signalling. Upstream kinase analysis suggested reduced activation of MNK2 and FAK (Fig. 6). To verify this, primary human myotubes from p.P50T/AKT2 carriers (n = 8 for phosphorylated (p) MNK2-Ser249 and pFAK-Tyr576/577 and n = 9 for peIF4E-Ser209 and pFAK-Tyr397) and controls (n = 8 for pMNK2-Ser249 and pFAK-Tyr576/577 and n = 7 for peIF4E-Ser209 and pFAK-Tyr397) were serum-starved for 2 h and stimulated with 0 and 100 nmol/L insulin, followed by Western blot analysis.
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