Caspian Harding on Muck Rack

Caspian Harding

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Articles

Programmable multistep CRISPR gene activation via control of RNA polymerase III termination

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By Anupama K Puppala, Emese Pataki, Kristen Vogt, Andrew Nielsen, Maureen Regan, Georgina E Mancinelli, Stephen Arnovitz, Nikolas G. Balanis, Ryan Clarke, Bradley J. Merrill, Caspian Harding, Michaele McGregor
| Science Magazine Verified
Abstract Although genomes encode instructions for mammalian cell differentiation with rich syntactic relationships, existing methods for genetically programming cells have only modest capabilities for stepwise gene regulation. Here, we develop a sequential genetic system that transcriptionally activates endogenous genes in a preprogrammed, stepwise manner.
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