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Catherine Barnier on Muck Rack

Catherine Barnier

As seen in: BioRxiv
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Articles

Regulation of the Drosophila transcriptome by Pumilio and CCR4-NOT deadenylase

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By Nathan D. Elrod, Hua Luo, Rebecca J. Haugen, Catherine Barnier
| BioRxiv
Abstract The sequence-specific RNA-binding protein Pumilio controls development of Drosophila; however, the network of mRNAs that it regulates remains incompletely characterized. In this study, we utilize knockdown and knockout approaches coupled with RNA-Seq to measure the impact of Pumilio on the transcriptome of Drosophila cells. We also used an improved RNA co-immunoprecipitation method to identify Pumilio bound mRNAs in Drosophila embryos.
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