Chabname Ghassemi Nedjad

Abstract A challenging problem in microbiology is to determine nutritional requirements of microorganisms and culture them, especially for the microbial dark matter detected solely with culture-independent methods. The latter foster an increasing amount of genomic sequences that can be explored with reverse ecology approaches to raise hypotheses on the corresponding populations.

INTRODUCTION The roles of microbiomes in the environment and in health-associated, agricultural or biotechnological applications are widely acknowledged (Foundation et al., 2022; Peixoto et al., 2022; Wilmes et al., 2022). These assemblies of microorganisms are governed by the interactions they have with their environment, and by those occurring among species (Zengler & Zaramela, 2018).