Charlly Kao

Abstract Recent advances in long-read single-cell transcriptome sequencing (lr-scRNA-Seq) enable full-length isoform profiling at single-cell resolution. We present SCOTCH (Single-Cell Omics for Transcriptome CHaracterization), an end-to-end, platform-independent pipeline for isoform characterization from lr-scRNA-Seq data, supporting Nanopore and PacBio sequencing as well as 10X Genomics and Parse Biosciences protocols.

Abstract Recent development involving long-read single-cell transcriptome sequencing (lr-scRNA-Seq) represents a significant leap forward in single-cell genomics. With the recent introduction of R10 flowcells by Oxford Nanopore, we propose that previous computational methods designed to handle high sequencing error rates are less relevant, and that the traditional approach using short reads to compile "barcode space" (candidate barcode list) to de-multiplex long reads are no longer necessary.

1 ACETYLCHOLINE (ACh) IN THE NERVOUS AND NON-NEURONAL CHOLINERGIC SYSTEMS (NNCS) ACh is a crucial neurotransmitter in both the central and peripheral nervous systems, responsible for transmitting signals between nerve cells and between nerves and muscles.1, 2 In the central nervous system (CNS), it affects various brain areas where it modulates neuronal excitability and plasticity, and is involved in cognitive functions like memory and attention.1, 3 In the peripheral nervous system, it plays...