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Experimental Procedures The coding sequences of PC isoform of Drosophila CaMKII (NP_524635) and Rattus norvegicus (NM_012920.1) a gift from the Lisman Lab at Brandeis University, were amplified via PCR from their respective plasmids and modified to include a 9-amino acid linker (GASGASGAS) at the N-terminal domain of the protein. The pSNAP-tag (m) Vector (#101135) obtained from Addgene was linearized using the PpUMI restriction enzyme.
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