Cintia Gomez Limia on Muck Rack

Cintia Gomez Limia

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Articles

Sequential, chromosome‐specific glutamine synthetase double knockout with Cas‐CLOVER establishes enhanced CHO platforms for cell line development

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By Cintia Gomez Limia, Daniel Machado, Valeriya Steffey, Thomas Franklin Hart, Mark McHargue, Corey O. Brizzee, Jack Crawford, Valeriya Steffey
| AIChE Journal Verified
1 INTRODUCTION Chinese hamster ovary (CHO) cells are the dominant expression system for recombinant proteins, particularly therapeutic antibodies, owing to their robust growth in suspension, adaptability to chemically defined media, capacity for human-compatible glycosylation, and high yield in fed-batch cultures.1, 2 CHO cells serve as the cornerstone of cell line development (CLD) platforms for the production of life-saving therapeutics such as pembrolizumab (Keytruda®),3 adalimumab...
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