Claudia Höbartner
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A magic methyl effect in the active site of a methyltransferase ribozyme
Abstract The chemical richness of RNAs is greatly enhanced by post-transcriptional modifications with RNA methylation as the most prominent type. RNA modifications modulate the stability, folding and interaction pattern of RNA molecules. Furthermore, emerging data suggests RNA modifications also directly regulate the activity of catalytic RNA molecules, i.e., ribozymes.
tRNA as an assembly chaperone for a macromolecular transcription-processing complex - Nature Structural & Molecular Biology
Abstract Transfer RNAs (tRNAs) are widely recognized for their role in translation. Here, we describe a previously unidentified function of tRNA as an assembly chaperone. During poxviral infection, tRNAGln/Arg lacking the anticodon mcm5s2U34 modification is specifically sequestered from the cellular tRNA pool to promote formation of a multisubunit poxviral RNA polymerase complex (vRNAP).
Ribozyme‐catalyzed site‐specific labeling of RNA using O6‐alkylguanine SNAP‐tag substrates
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