Colette A. Makara

Abstract Click to copy section linkSection link copied! Described is the use of photocatalytic and enzymatic activation of rapid bioorthogonal chemistry for cell surface proximity labeling. Current approaches to proximity labeling rely on the activation of reactive intermediates (e.g., carbenes, nitrenes, radicals, electrophiles), whose half-life is regulated by uncontrollable quenching by water and native biomolecules in the cell or media.