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Congwang Ye

As seen in: ACS Publications, BioRxiv, Chemistry World
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Articles

Real-Time Affinity Measurements of Proteins Synthesized in Cell-Free Lysate Using Fluorescence Correlation Spectroscopy

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By Chao Liu, Steven Hoang-Phou, Congwang Ye, Emma Laurence
| ACS Publications
ArticleApril 30, 2025 Chao Liu Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, California 94550, United States Steven A. Hoang-Phou Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, California 94550, United States Congwang Ye Materials Engineering Division, Lawrence Livermore National Laboratory, Livermore, California 94550, United States Emma J.
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Real-Time Affinity Measurements of Proteins Synthesized in Cell-Free Lysate Using Fluorescence Correlation Spectroscopy

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By Chao Liu, Steven Hoang-Phou, Congwang Ye, Emma Laurence
Open in Who Shared Issue with article?

Real-time affinity measurements of proteins synthesized in cell-free lysate using fluorescence correlation spectroscopy

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By Chao Liu, Steven Hoang-Phou, Congwang Ye, Emma Laurence
| BioRxiv
Abstract Rapid, high throughput measurements of biomolecular interactions are essential across medicine and bioscience. Traditional methods for affinity-screening proteins require a long and costly process involving cell-based expression, purification, and titration of multiple concentrations to arrive at a binding curve.
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