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Open-source Photobleacher for Fluorescent Imaging of Large Pigment-Rich Tissues

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By Tatsuya Murakami, Nick Belenko, Griffin Dennis, Cuidong Wang
| biorxiv.org
Abstract Fluorescent imaging enables visualization of the specific molecules of interest with high contrast, and the use of multiple fluorophores in a single tissue sample allows visualization of complex relationships between biological molecules, cell types, and anatomy. The utility of fluorescent imaging in human tissue has been limited by endogenous pigments that can block the light path or emit an autofluorescence, thereby interfering with the specific imaging of target molecules.
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