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Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits

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By Biomedical Imaging, D Barson, AS Hamodi, X Shen
| BioRxiv
Abstract Spontaneous and sensory-evoked activity propagates across spatial scales in the mammalian cortex but technical challenges have generally precluded establishing conceptual links between the function of local circuits of neurons and brain-wide network dynamics. To solve this problem, we developed a method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and mesoscopic widefield calcium imaging of the entire cortical mantle in awake, behaving mice.
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