Dana Motabar

Abstract Protein function relies on sequence, folding and post-translational modification and molecular measurements are commonly used to reveal these structural features. Here, we report an alternative approach that represents these molecular features as readily measurable electronic patterns and validate this experimental approach by detecting structural perturbations commonly encountered during protein biomanufacturing.

1 INTRODUCTION The development process for antibody therapeutics is both costly and time-consuming owing to the complexities of optimizing host cell lines, bioreactors, processing conditions, and product characterization (Rodrigues et al., 2010; Shukla et al., 2017; Steinmeyer & McCormick, 2008). Measurement tools that rapidly and accurately assess the production process and product quality are of great interest as they can speed development times.

Abstract Protein function relies on sequence, folding, and post-translational modification, and molecular measurements are commonly used to reveal these structural features. Here, we report an alternative approach that represents these molecular features as readily measurable electronic patterns and validate this experimental approach by detecting structural perturbations commonly encountered during protein biomanufacturing.