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Daniel Weisgerber

As seen in: Royal Society of Chemistry, JoVE- Journal of Visualized Experiments
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Articles by Daniel Weisgerber

Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP

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By Daniel Weisgerber, Ca Usa, Krzysztof Langer
| Royal Society of Chemistry Verified
Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP Daniel W. Weisgerber, a Krzysztof Langer,a Venice Servellita,b Peng Xu,a Charles Y. Chiubc and Adam R. Abate *ad Abstract Viral load quantitation is useful in clinical point-of-care settings to assess the status of patients with infectious disease, track response to treatment, and estimate infectiousness. However, existing methods for quantitating viral loads are complex and difficult to integrate into these settings.
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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By Benjamin Demaree, Daniel Weisgerber
| JoVE- Journal of Visualized Experiments
The SiC-seq experimental workflow contains 3 PDMS microfluidic devices fabricated using a soft lithography procedure (Figure 1). A co-flow dropmaker (Figure 3A) generates 25 µm of digital barcode droplets for labeling genomic DNA with a unique single-cell identifier. The barcode oligonucleotides consist of a 15 bp degenerate sequence flanked by PCR handles for amplification (Table 2, BAR primer).
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