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I grew up in rural southeastern Australia, which is an area of stunning natural beauty. Our house was set in natural bushland with lots of space to ride motor bikes and go exploring. I was fortunate to have parents who instilled in me a sense of discovery and of nature. I was fascinated by the natural life-forms around me — especially the things I could get up close to. I caught small bugs and critters with my net to keep in an aquarium.
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Abstract Methods that assay protein foldedness with proteomics have generated censuses of apparent protein folding stabilities in biological milieu. However, different censuses poorly correlate with each other. Here, we show that the reason for this is that methods targeting foldedness through monitoring amino acid sidechain reactivity also detect changes in conformation and ligand binding, which can be a substantial fraction of the data.
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