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Endogenous and exogenous immunoprecipitations Harvested cells were washed twice with cold PBS and washed pellets resuspended with Buffer A (25mM HEPES pH 7.6, 5mM MgCl2, 25mM KCl, 0.05mM EDTA, 10% (v/v) glycerol, 0.1% (v/v) NP40, 1mM DTT and 1mM PMSF) and kept on ice for 10 minutes. Samples were centrifuged at 1,500rpm for 10 minutes at 4°C and the supernatant was discarded.
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