David Bedwell

How can someone be there one moment and then gone forever the next? I mean obviously that’s life and death and just how things work but it’s a monumentally strange concept and such a mind blowing thing to experience. Like you spend day after day with this person and then suddenly they’re just not there anymore? And you’ll never see them again. Never speak to them again. Just nothing. Gone. The even weirder thing is that everything else keeps going.

Side note: these blogs are really just a coping mechanism I’m experimenting with. Allowing myself to get the thoughts and feelings out so I don’t go entirely crazy. It’s not some endeavour to go viral or have a popular blog. But I’d also like to be able to share with people I haven’t had a chance to talk to, or even strangers who may be going through the same thing. So read or don’t read. It’s okay.

Abstract Protein synthesis terminates when a stop codon enters the ribosome’s A-site. Although termination is efficient, stop codon readthrough can occur when a near-cognate tRNA outcompetes release factors during decoding. Seeking to understand readthrough regulation we used a machine learning approach to analyze readthrough efficiency data from published HEK293T ribosome profiling experiments and compared it to comparable yeast experiments.