McGill BME is Canada's first department of biomedical engineering. A global leader in research, education, and translation to the medical technology industry.
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Articles by David Juncker
Co-enrichment of proteins in extracellular vesicles
Abstract Extracellular vesicles are cell-derived secretions that mediate tissue homeostasis and intercellular communication through their diverse cargos, including proteins. Distinct extracellular vesicle biogenesis pathways suggest specific association and co-enrichment of proteins sharing a biogenesis pathway, and non-association and co-depletion of proteins segregated into distinct pathways. Yet these associations elude conventional protein expression or co-expression measurements.
Nanoporous PEGDA ink for High-Resolution Additive Manufacturing of Scaffolds for Organ-on-a-Chip
Abstract Polydimethylsiloxane (PDMS), commonly used in organ-on-a-chip (OoC) systems, faces limitations in replicating complex geometries, hindering its effectiveness in creating 3D OoC models. In contrast, poly(ethylene glycol)diacrylate (PEGDA-250), favored for its fabrication ease and resistance to small molecule absorption, is increasingly used for 3D printing microfluidic devices. However, its application in cell culture has been limited due to poor cell adhesion.
Digital Manufacturing of Functional Ready‐to‐Use Microfluidic Systems
Research Article This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as https://doi.org/10.1002/adma.202303867 Abstract Digital manufacturing (DM) strives for the seamless manufacture of a functional device from a digital file.
Digital Manufacturing of Functional Ready-to-Use Microfluidic Systems
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Microfluidic chain reaction of structurally programmed capillary flow events - Nature
Abstract Chain reactions, characterized by initiation, propagation and termination, are stochastic at microscopic scales and underlie vital chemical (for example, combustion engines), nuclear and biotechnological (for example, polymerase chain reaction) applications1,2,3,4,5. At macroscopic scales, chain reactions are deterministic and limited to applications for entertainment and art such as falling dominoes and Rube Goldberg machines.
Opinion | Why we don’t have enough rapid tests — and what we can do about it
Health Canada has a critical role to play, and must adapt to a changing world to allow manufacturers to participate actively in stopping the pandemic. By David Juncker Don VinhContributors Thu., Jan. 6, 2022timer3 min. read As COVID19 is again spreading uncontrollably, millions of Canadians are clamouring for rapid self-tests. The Omicron variant finally swept aside the suspicions of provincial public health agencies toward rapid tests, as they now hand out the few they have.
Opinion: Rapid antigen tests needed for a safe reopening
In many other places, rapid antigen tests are readily available, but Quebec still requires they be performed by a health professional. Author of the article: Marie-Pascale Pomey and David Juncker Stratford & District Chamber of Commerce general manager Eddie Matthews tests himself with one of the free rapid antigen screening tests now available to small- and medium-sized businesses throughout Ontario through that province's new COVID-19 Rapid Screening Initiative.
Duplexed aptamers: history, design, theory, and application to biosensing
Duplexed aptamers: history, design, theory, and application to biosensing Jeffrey D.
Two-level submicron high porosity membranes (2LHPM) for the capture and release of white blood cells (WBCs)
Two-level submicron high porosity membranes (2LHPM) for the capture and release of white blood cells (WBCs) J. Alejandro Hernández-Castro, Kebin Li, Jamal Daoud, David Juncker and Teodor Veres Abstract A method by modifying vacuum assisted UV micro-molding (VAUM) process is proposed for the fabrication of polymer nano-in-micropore membranes (NaiMM).
Ensemble multicolour FRET model enables barcoding at extreme FRET levels
Quantitative models of Förster resonance energy transfer (FRET)—pioneered by Förster—define our understanding of FRET and underpin its widespread use. However, multicolour FRET (mFRET), which arises between multiple, stochastically distributed fluorophores, lacks a mechanistic model and remains intractable. mFRET notably arises in fluorescently barcoded microparticles, resulting in a complex, non-orthogonal fluorescence response that impedes their encoding and decoding.
Capillary microfluidics in microchannels: from microfluidic networks to capillaric circuits
Microfluidics offer economy of reagents, rapid liquid delivery, and potential for automation of many reactions, but often require peripheral equipment for flow control. Capillary microfluidics can deliver liquids in a pre-programmed manner without peripheral equipment by exploiting surface tension effects encoded by the geometry and surface chemistry of a microchannel. Here, we review the history and progress of microchannel-based capillary microfluidics spanning over three decades.
Comprehensive profiling of the ligand binding landscapes of duplexed aptamer families reveals widespread induced fit
We introduce ACE-Scan, which differentiates between conformational selection and induced fit ligand-binding pathways in DAs using a non-equilibrium surface-based assay15 (Fig. 1 and Methods section).
Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
The assay procedure for both single and double transfer methods is shown in Figure 1. In single transfer, the cAbs are spotted directly on the assay slide and the dAbs are transferred onto the assay slide upon use in a mirror pattern of the cAbs (Figure 1a). Only one transfer procedure is required, but this method suffers from misalignment between the two microarrays, mainly caused by the angular misalignment between the slide and the inkjet gantry (Figure 2).
Fabrication of large-area polymer microfilter membranes and their application for particle and cell enrichment
A vacuum assisted UV micro-molding (VAUM) process is proposed for the fabrication of freestanding and defect-free polymer membranes based on UV-curable methacrylate polymer (MD 700).
Complementary Oligonucleotides Regulate Induced Fit Ligand Binding in Duplexed Aptamers
Duplexed aptamers (DAs) are engineered by hybridizing an aptamer-complementary element (ACE, e.g. a DNA oligonucleotide) to an aptamer; to date, ACEs have been presumed to sequester the aptamer into a non-binding duplex state, in line with a conformational selection-based model of ligand binding. Here, we uncover that DAs can actively bind a ligand from the duplex state through an ACE-regulated induced fit mechanism.
Complementary oligonucleotides regulate induced fit ligand binding in duplexed aptamers
Duplexed aptamers (DAs) are engineered by hybridizing an aptamer-complementary element (ACE, e.g. a DNA oligonucleotide) to an aptamer; to date, ACEs have been presumed to sequester the aptamer into a non-binding duplex state, in line with a conformational selection-based model of ligand binding. Here, we uncover that DAs can actively bind a ligand from the duplex state through an ACE-regulated induced fit mechanism.
Autonomous Microfluidic Capillaric Circuits Replicated from 3D-Printed Molds
We recently developed capillaric circuits (CCs) – advanced capillary microfluidic devices assembled from capillary fluidic elements in a modular manner similar to the design of electric circuits (Safavieh & Juncker, Lab Chip, 2013, 13, 4180-4189). CCs choreograph liquid delivery operations according to pre-programmed capillary pressure differences with minimal user intervention.
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