Davide Dalla Torre

Abstract HIV-1 uses the metabolite inositol hexakisphosphate (IP6) as a host factor to assemble its capsid, but whether this strategy is unique to lentiviruses or represents a common feature of retroviral capsids remains unclear. Here we show that IP6 binding is conserved across diverse retroviruses but occurs through distinct capsid sites and mechanisms, and influences viral behaviour.

Motivation and Original Contribution of the Present Work In the framework of in silico models for COC experiments, our current study aims to extend the previous version of the model we already developed [25]. Since our approach is based on relatively simple computational algorithms (cellular automata and agents), it makes it easier to reproduce the chip geometry with respect to other possible approaches like those mentioned above.

Plasmid constructs design and cloning DmCas7-11 used in the present study was coexpressed together with crRNA in Escherichia coli BL21(DE3) from a single plasmid containing the kanamycin resistance gene. CrRNA is processed to shorter crRNA molecules on coexpression with Ca7-11. To construct the plasmid for coexpression, a coding sequence of full-length DmCas7-11 codon optimized for E.