David Papp

1 INTRODUCTION Members of the ascomycete fungal genus Cryphonectria (Sacc.) Sacc. & D. Sacc. are significant forest pathogens infecting trees in the Fagaceae and Betulaceae families. The most infamous and destructive species of the genus is C. parasitica (Murrill) M.E. Barr, which is the causal agent of chestnut blight and has inflicted significant harm on the chestnut stands in Europe and North America (Rigling & Prospero, 2018).

Abstract Myotonic Dystrophy Type 2 (DM2) is a genetic disease caused by expanded CCTG DNA repeats in the first intron of CNBP. The number of CCTG repeats in DM2 patients ranges from 75-11,000, yet little is known about the molecular mechanisms responsible for repeat expansions or contractions. We developed an experimental system in Saccharomyces cerevisiae that enables selection of large-scale contractions of (CCTG)100 within the intron of a reporter gene and subsequent genetic analysis.

Investigation of cyclic IMS settings influencing the observed ion heating In the literature, it has already been highlighted that voltage settings of the cyclic IMS instrument may significantly influence ion transmission and activation for large biomolecular systems.69 Correspondingly, we aimed to highlight cyclic IMS parameters that may have an impact on the observed peptide ion heating. To this end, we investigated the effect of several IMS settings on peptide fragmentation.