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BCR::ABL1 expression in chronic myeloid leukemia cells in low oxygen is regulated by glutamine via CD36-mediated fatty acid uptake - Cancer Cell International
The K562 (RRID: CVCL_0004) and KCL22 (RRID: CVCL_2091) BCR::ABL1-positive cell lines derived from CML patients in blast crisis were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 2 mM Gln (Euroclone, Milan, Italy) and incubated at 37 °C in 5% CO2 atmosphere.
Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity
Histological examination of skeletal muscle biopsies from human cadavers at 6–17 days post mortem showed severe autolytic alterations with oedema compared with normal tissue (57–95 years old (y.o.); n=16; Table 1). However, examination with markers showed the presence of muscle stem (satellite) cells (CD56+/N-CAM+; Fig. 1a,d and insets)8. Strikingly, as observed with freshly isolated human muscle stem cells (Fig.
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