Dianwen Han

1 Introduction Breast cancer represents the second leading cause of cancer incidence among women globally.[1] However, the exact mechanisms leading to its occurrence and development remain poorly understood.

The breast cancer tissues and normal tissues were obtained from breast cancer patients undergoing surgery in Qilu Hospital of Shandong University. Survival time refers to the time from operation date to the date of last follow-up or death. This research was approved by the Ethical Committee of Qilu Hospital of Shandong University, and informed consents from all of patients were obtained. Total RNA was isolated using TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol.