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Domen Zafred on Muck Rack

Domen Zafred

As seen in: BioRxiv
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Articles

Efficient overexpression and purification of SARS-CoV-2 Nucleocapsid proteins in Escherichia coli

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By Emma L Brudenell, Manoj B Pohare, Domen Zafred, Janine Phipps
| BioRxiv
Abstract The fundamental biology of Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (Ncap), its use in diagnostic assays and its potential application as a vaccine component have received considerable attention since the outbreak of the Covid19 pandemic in late 2019. Here we report the scalable expression and purification of soluble, immunologically active, SARS-CoV-2 Ncap in Escherichia coli.
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