Einar Halldórsson

Abstract Cellular organization in the form of biomolecular condensates is a fundamental regulatory mechanism across all forms of life. Formation of condensates relies on multivalent interactions that are often weak and transient, making them challenging to study experimentally. We have developed Condensate Partitioning by mRNA-Display (CPmD) to measure these interactions from the partition free energies of peptides and nucleic acids into reconstituted condensates.

Abstract Intrinsically disordered protein regions facilitate cellular organization through phase separation into biomolecular condensates. However, the molecular interactions driving this process remain poorly understood because of experimental limitations. Here, we advance experimental throughput by several orders of magnitude by developing Condensate Partitioning by mRNA-Display (CPmD).