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Biological Studies Biological experiments were performed on MDA-MB-231, MCF-7, Hep G2, BJ fibroblasts, and BV-2 cells. Cell Viability Assay: Cells were seeded in 96-well plates and incubated for 24 h at 37 °C with 5% CO₂. Diblock copolymer stock solutions were prepared in PBS, diluted in DMEM/F-12 medium with 10% FBS and 1% P/S, sterilized through a 0.2 µm filter, and added to wells at final concentrations of 0–1 mg mL−1.
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