Eliza Barańska

Introduction Click to copy section linkSection link copied! Fluorescence microscopy allows precise visualization of cellular structures and dynamic processes, with specificity achieved through targeted fluorescent labeling of biomolecules. This enables precise tracking of molecular interactions and cellular responses, which is crucial for understanding complex biological processes.

Photophysical Properties of Trz+Fluorophores The absorption properties of Trz+1 are significantly different from the tetrazine analogue MeTzNH2 which can be inferred from the distinct colors of their solutions ( Figure 3 A, 3 B “before”). The aqueous solution of tetrazine has the typical pink-reddish color (λ max = 521 nm), while the Trz+1 solution is significantly darker with an intense panchromatic absorption between ca. 350 and 700 nm (λ max at 426 and 523 nm, Figure 3 A, 3 B).