Ellen Kazumi Okuda

Abstract Small subcellular organelles orchestrate key cellular functions. How biomolecules are spatially organized within these assemblies is poorly understood. Here, we report an automated super-resolution imaging and analysis workflow that integrates confocal microscopy, morphological object screening, targeted 3D super-resolution STED microscopy and quantitative image analysis.

The nucleus of eukaryotic cells is compartmentalized into different membraneless organelles (MLOs) with important functions. Nuclear speckles are enriched in RNA-binding proteins (RBPs) and splicing factors with mixed-charge domains, whereas paraspeckles contain RBPs with prion-like domains that are spatially organized by the scaffold RNA NEAT1. Both MLOs coordinate the nuclear stress response and are found in close proximity to each other, but have not been found to fuse.

Citation Benjamin ArnoldRicarda J. RieggerEllen Kazumi OkudaIrena SliškovićMario KellerCem BakisogluFrançois McNicollKathi ZarnackMichaela Müller-McNicoll; hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates. J Cell Biol 5 February 2024; 223 (2): e202304030. doi: https://doi.org/10.1083/jcb.202304030 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex