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Elyse A Shoppell on Muck Rack

Elyse A Shoppell

As seen in: BioRxiv
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Articles

Proof of concept study deploying CRISPR inhibition and activation opens key avenues for systematic biological exploration in zebrafish.

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By Nelson B. Barrientos, Elyse A Shoppell, Rachel J. Boyd, Valeria C. Culotta
| BioRxiv
Abstract The application of CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) technologies in zebrafish has the potential to expand its capacity for the study of gene function significantly. We report proof-of-principle data evaluating transient expression of a codon optimized CRISPRi/a system for zebrafish across established pigmentary and growth phenotypes.
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Optimized CRISPR inhibition and activation opens key avenues for systematic biological exploration in zebrafish.

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By Nelson B. Barrientos, Rachel J. Boyd, Valeria C. Culotta, Elyse A Shoppell
| BioRxiv
Abstract The application of CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) technologies in zebrafish has the potential to expand its capacity for the study of gene function significantly. We have developed a codon optimized CRISPRi/a for zebrafish; here we provide proof-of-principle data across established pigmentary and growth phenotypes. We established a zebrafish codon-optimized cas9 gene, harboring mutations D10A and D839A to render the protein catalytically inactive (dCas9).
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