Emanuela Gionfriddo

The “autosampler” is an indispensable component of modern high-performance liquid chromatography (HPLC) systems. Contemporary technology enables automated, rapid, and precise injections of samples with very little carryover. However, the rotor/stator assembly at the heart of injection valves used in these samplers is also a weak point. Routine wear of the rotor can lead to leaks and release debris into the mobile phase, leading to obstructions downstream from the injection valve.

Researchers evaluating leaf and tissue culture (callus) extracts and corresponding volatile oils from Eucalyptus camaldulensis Dehn (Family Myrtaceae), extensively exploited for its anti-inflammatory efficacy, subjected the methanolic extracts from both plant sources to liquid chromatography–mass spectrometry (LC–MS). Their findings underscore the potential of in vitro tissue culture as a biotechnological tool for amplifying the yield and bioactivity of secondary metabolites in E. camaldulensis.

News|Articles|February 5, 2026 LCGC International January/February 2026 Volume 3 Issue 1 Pages: 12–14 SEC calibration links elution volume/time to polymer hydrodynamic volume using standards, requiring model-form selection that preserves predictive molecular-weight distribution fidelity. Least-squares regression minimizes summed squared residuals, and R² quantifies explained variance but systematically favors higher-parameter polynomials regardless of true signal.