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Genome Biology volume 23, Article number: 19 (2022) Cite this article Abstract Background Next-generation sequencing (NGS) can identify mutations in the human genome that cause disease and has been widely adopted in clinical diagnosis. However, the human genome contains many polymorphic, low-complexity, and repetitive regions that are difficult to sequence and analyze.
Highlights • Drop-seq reveals a strong cancer cell heterogeneity in PyMT mammary tumors • Elf5 redefines cancer cell states toward basal-like with pro-metastatic features • PyMT/Elf5 tumors show cellular and molecular characteristics of involution mimicry • Involution CAFs in PyMT/Elf5 tumors interconnect the TME and cancer cells Summary Basal breast cancer is associated with younger age, early relapse, and a high mortality rate.
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