Cell Reports

Keywords intercellular communication cryo-electron tomography cryo-focused ion beam milling bacterial multicellularity heterocyst formation cyanobacteria cellular differentiation diazotrophic growth fluorescence recovery after photobleaching Research topic(s) CP: microbiology CP: molecular biology Introduction Multicellularity relies fundamentally on cell-cell communication.1 In filamentous cyanobacteria such as Nostoc sp.

Results To comprehensively characterize the molecular and morphological changes to mitochondria and the ETC (Figure 1A) during embryogenesis, we used zebrafish as a model system given the accessibility of externally developing embryos for detailed biochemical and functional studies.

Keywords ASS1 ubiquitination OTUB2 AMPK DDIT3 C/EBPα arginine deprivation therapy ADT Research topic(s) CP: cancer CP: molecular biology Introduction Tumor cells rely on extensive nutrient consumption, such as glucose, nucleic acids, lipids, and amino acids to fuel rapid proliferation, invasion, immune evasion, and angiogenesis.1 Arginine, a semi-essential amino acid, plays dual roles in cellular metabolism: it serves as a critical intermediate in the urea cycle and as a primary substrate for...

Keywords cortical layers graphs algorithm subnetworks columns volumetric calcium imaging multi-layers analysis Research topic(s) CP: neuroscience Introduction Although cortical columns have long been proposed as the neocortex’s elementary processing units,1 it remains poorly understood how their three-dimensional (3D) functional pathways are spatially organized across layers.

Results To systematically define the function of ERK1 and ERK2 in hESCs, we utilized stepwise CRISPR-Cas9n genetic editing methods to deplete ERK1 and ERK2 in hESCs (Figure 1A and S1A). Briefly, CRE hESCs constitutively expressing ERT2CREERT2 served as control cells. The inducible KO (iKO) of ERK1 or ERK2 was achieved by 4-hydroxytamoxifen (4OHT)-mediated recombination of LoxP sites. An ERK1/2 iKO line (ERK1Δ/Δ; ERK2fl/-) was generated from the ERK1 KO line.

Keywords chromatin epigenetics Polycomb embryogenesis Drosophila Research topic(s) CP: molecular biology CP: genomics Introduction Multicellular organisms employ Polycomb group (PcG) proteins, which function in complexes including Polycomb repressive complex 1 (PRC1) and Polycomb repressive complex 2 (PRC2), to maintain patterns of gene silencing.1,2,3 PRC2 methylates histone H3 at lysine 27 (H3K27), and both di- and tri-methylated H3K27 (H3K27me2 and H3K27me3) contribute to negative...

Generation of Pum1 and/or Pum2 knockout mESCs Paired guide RNAs (gRNAs) targeting specific sequences within exon 12 of Pum1 and exon 6 of Pum2, with a spacing of 5–30 bp (Figure S1A), were designed using the Benchling-sgRNA online tool (https://www.benchling.com/crispr/). Each sgRNA pair was cloned into a pGL3-U6-sgRNA-PGK-puromycin vector (Addgene plasmid Cat# 51133).

Keywords beaten path family sidestep family Drosophila melanogaster cell surface receptor axon guidance neuromuscular junction protein structure Research topic(s) CP: molecular biology CP: neuroscience Introduction The development of the neuromuscular system is dependent on a molecular landscape controlled by cell-surface and secreted proteins.

ArticleVolume 45, Issue 7117671Open access Affiliations & Notes 1Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China 2State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China 3Archaeal Biology Center, Synthetic Biology Research Center, Shenzhen Key Laboratory of Marine Microbiome Engineering, Key Laboratory of Marine Microbiome Engineering of Guangdong...

Keywords histone acetylation HDAC Rpd3 yeast epigenetics metabolism deacetylation Research topic(s) CP: molecular biology Introduction Microorganisms exhibit remarkable plasticity in adapting to changing environments by reprogramming their metabolism and gene expression to optimize survival and growth.1,2 In the budding yeast Saccharomyces cerevisiae, glucose serves as the preferred carbon source and drives rapid fermentative growth.3,4 When glucose becomes scarce, yeast cells switch to...